Composite

Part:BBa_K4378012:Design

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)


Expression cassette for tphA1/TPADO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2055
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2283
    Illegal NgoMIV site found at 2794
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1651


Design Notes

Note that each basic part has been codon optimized for E. coli expression.

Source

Composite, see each basic part for references.

References